ABSTRACT
Five clones of axenic E. histolytica (HMI) grown as discrete colonies in semisolid agar medium were adapted in liquid medium and labelled as HMI-C121, HMI-C131, HMI-C143, HMI-C144 and HMI-C145. Isoenzymes of these 5 clones of E. histolytica (HMI) were investigated in starch gel electrophoresis. There were no differences in the electromobility of maleate NADP oxidoreductase and glucosephosphoisomerase amongst the five clones and uncloned cultures of axenic E. histolytica. The relative electromobility (rf) of a single phosphoglucomutase (PGM) band of uncloned Mexican E. histolytica (HMI) and Indian axenic E. histolytica (KCG: 0986: 11) cultures and cloned E. histolytica HMI-C121, HMI-C145 was 0.087 while a single PGM band of uncloned E. histolytica (NIH: 200) and cloned E. histolytica HMI-C131, HMI-C143 and HMI-C144 cultures had rf of 0.075. Isoenzyme characterization of four cloned HMI-C121, HMI-C131, HMI-C143, HMI-C144 cultures of axenic E. histolytica (HMI) revealed existence of three bands of hexokinase (HK). The additional third band of HK was located close to the place of application of lysate and had rf ranging from 0.11-0.14. The data indicated that parent axenic E. histolytica (HMI) consisted of several populations and each population expressed different isoenzyme pattern without an association of amoebic cultures with any bacterial species.
Subject(s)
Animals , Clone Cells , Entamoeba histolytica/enzymology , Glucose-6-Phosphate Isomerase/analysis , Hexokinase/analysis , Isoenzymes/analysis , NADH, NADPH Oxidoreductases/analysis , Phosphoglucomutase/analysisABSTRACT
A fosfoglucomutase placental (locos PGM1, PGM2 e PGN3) foi investigada em uma amostra de 442 recém-nascidos (54% Brancos e 46% Negros) da populaçäo de Porto Alegre. As freqüências gênicas observadas foram: Branco: PGMI*2 = 0.24, PGM2*1 = 1.00 e PGm3*2 = 0.32; Negros: PGM1*2 = 0.22, PGM2*1 = 1.00 e PGM3*2 = 0.45. Detectou-se entre os negros um indivíduo heterozigoto para um possível alelo nulo no loco PGM1. Näo foi verificada associaçäo nas distribuiçöes conjuntas entre os locos PGN1 e PGM3, bem como, näo se encontrou efeito dos fenótipos de PGN1 e PGM3 no desenvolvimento do feto ou da placenta
Subject(s)
Infant, Newborn , Humans , Gene Frequency , Phosphoglucomutase/analysis , Placenta/enzymology , Polymorphism, Genetic , Brazil , PhenotypeABSTRACT
Isoenzyme patterns of adult Malaysian Schistosoma, S. mekongi and S. japonicum strains were analysed by isoelectric focusing (IEF) in polyacrylamide gel. Enzyme patterns obtained from Malaysian Schistosoma homogenates differed from those of S. mekongi and S. japonicum strains. Malaysian Schistosoma was found to differ from S. japonicum by 8 enzymes, namely phosphoglucomutase, phosphoglucoisomerase, malate dehydrogenase, acid phosphatase, hydroxy-butyrate dehydrogenase, hexokinase and alkaline phosphatase, and from S. mekongi by phosphoglucomutase, malate dehydrogenase, aldolase and alkaline phosphatase. These results and the distinct biology of the parasite suggest that Malaysian Schistosoma is a new species in the S. japonicum complex.